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My sister is determined. ________ she has made up her mind, nothing can change it.

A.ThoughB.OnceC.UnlessD.Until

B

解析试题分析:“一旦”下定决心,什么也不能改变(她的决心)。once: as soon as, when, 引导时间状语。A项引导让步状语从句;C项引导条件状语从句;D项引导时间状语从句,但是要求主句动词为延续性动词或动词的否定形式。故B项正确。
考点:状语从句。

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